PIGA Knockout cell line (K-562)

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PIGA Knockout cell line (K-562) Order

PIGA Knockout cell line (K-562)
Catalog#
YKO-H109
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
PIGA Knockout cell line (K-562)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
PIGA Knockout cell line (K-562)
Cell Morphology
Lymphocyte-like,suspension
Passage ratio
1：3-1：5
Species
Human
Gene Name
PIGA
Gene ID
5277
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%IMDM+10%FBS
Accession
CVCL_E0WN(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 2 (based on transcript PIGA-201)
Validation: PCR+Sanger Sequencing
PIGA Knockout cell line (K-562) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:K-562		 STR Info (Cell bank)
Cell Line:K-562
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 8888
D7S820 911911
D16S539 11121112
VWA 16161616
TH01 9.39.39.39.3
AMEL XXXX
TPOX 8989
CSF1PO 910910
D12S391 2323
FGA 2124
D2S1338 1717
D21S11 293031.0
D18S51 1516
D8S1179 1212
D3S1358 1616
D6S1043 1115
PENTAE 514
D19S433 1414.2
PENTAD 913
D1S1656 1516
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the PIGA gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the K-562 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, PIGA gene knockout PIGA Knockout cell line (K-562) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
PIGA Knockout cell line (K-562)
PIGA Knockout cell line (K-562)
Catalog#
YKO-H109
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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